XMRV werd verondersteld via de XPR1-receptor aan te haken bij cellen/"nieuwe slachtoffers"
(klik hier),
maar uit een recent gepubliceerde studie van Hewlett en kollega's
blijkt dat XMRV ook in staat is cellen te infekteren die géén XPR1-receptor "dragen".
De replikatie (vermenigvuldiging) van XMRV varieert in verschillende typen cellen.
Het blijft toch vreemd dat er zoveel aandacht is voor een "onschuldig" endogeen virus.
Nog los van de vraag hoe deze bevindingen te rijmen zijn met kontaminatietheorieën.
XMRV: Usage of receptors and potential co-receptors.
Virol J. 2011 Sep 6;8(1):423. doi:10.1186/1743-422X-8-423.
Haleyur Giri Setty MK, Devadas K, Ragupathy V, Ravichandran V, Tang S, Wood O, Gaddam DS, Lee S, Hewlett IK.
ABSTRACT:
BACKGROUND:
XMRV is a gammaretrovirus
first identified in prostate tissues of Prostate Cancer (PC) patients and
later in the blood cells of patients with Chronic Fatigue Syndrome (CFS).
Although XMRV is thought to use XPR1 for cell entry,
it infects A549 cells that do not express XPR1,
suggesting usage of other receptors or co-receptors.
METHODS:
To study the usage of
different receptors and co-receptors
that could play a role in
XMRV infection of
lymphoid cells and
GHOST (GFP-Human osteosarcoma) cells
expressing CD4
along with different chemokine receptors
including CCR1, CCR2, etc.,
were infected with XMRV.
Culture supernatants and cells
were tested for XMRV replication
using real time quantitative PCR.
RESULTS:
Infection and replication of
XMRV
was seen in a variety of
GHOST cells, LNCaP, DU145, A549 and Caski cell lines.
The levels of XMRV replication varied in different cell lines
showing differential replication in different cell lines.
However, replication in A549
which lacks XPR1 expression
was relatively higher than
DU145
but lower than, LNCaP.
XMRV replication varied
in GHOST cell lines
expressing CD4 and
each of the co-receptors CCR1 - CCR8 and Bob.
There was significant replication of XMRV
in CCR3 and Bonzo
although it is much lower
when compared to
DU145, A549 and LNCaP.
CONCLUSION:
XMRV replication
was observed in
GHOST cells that express CD4, and
each of the chemokine receptors
ranging from CCR1- CCR8 and Bob
suggesting that
infectivity in hematopoietic cells
could be mediated by use of these receptors.
PMID: 21896167
Samenvatting:
http://www.virologyj.com/content/8/1/423/abstract
Volledige tekst:
http://www.virologyj.com/content/pdf/1743-422X-8-423.pdf
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