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Hewlett:

XMRV

verschaft zich

niet alleen via XPR1

toegang tot de cel

 

 

 

 


 

 

 

XMRV werd verondersteld via de XPR1-receptor aan te haken bij cellen/"nieuwe slachtoffers" (klik hier), maar uit een recent gepubliceerde studie van Hewlett en kollega's

blijkt dat XMRV ook in staat is cellen te infekteren die géén XPR1-receptor "dragen".

 

De replikatie (vermenigvuldiging) van XMRV varieert in verschillende typen cellen.

 

 

Het blijft toch vreemd dat er zoveel aandacht is voor een "onschuldig" endogeen virus.

Nog los van de vraag hoe deze bevindingen te rijmen zijn met kontaminatietheorieën.

 

 


 

 

 

XMRV: Usage of receptors and potential co-receptors.

Virol J. 2011 Sep 6;8(1):423. doi:10.1186/1743-422X-8-423.

Haleyur Giri Setty MK, Devadas K, Ragupathy V, Ravichandran V, Tang S, Wood O, Gaddam DS, Lee S, Hewlett IK.

 

 

ABSTRACT:

 

 

BACKGROUND:

 

XMRV is a gammaretrovirus

first identified in prostate tissues of Prostate Cancer (PC) patients and

later in the blood cells of patients with Chronic Fatigue Syndrome (CFS).

 

Although XMRV is thought to use XPR1 for cell entry,

it infects A549 cells that do not express XPR1,

suggesting usage of other receptors or co-receptors.

 

 

METHODS:

 

To study the usage of

different receptors and co-receptors

that could play a role in

XMRV infection of

lymphoid cells and

GHOST (GFP-Human osteosarcoma) cells

expressing CD4

along with different chemokine receptors

including CCR1, CCR2, etc.,

were infected with XMRV.

 

Culture supernatants and cells

were tested for XMRV replication

using real time quantitative PCR.

 

 

RESULTS:

 

Infection and replication of

XMRV

was seen in a variety of

GHOST cells, LNCaP, DU145, A549 and Caski cell lines.

 

The levels of XMRV replication varied in different cell lines

showing differential replication in different cell lines.

 

However, replication in A549

which lacks XPR1 expression

was relatively higher than

DU145

but lower than, LNCaP.

 

XMRV replication varied

in GHOST cell lines

expressing CD4 and

each of the co-receptors CCR1 - CCR8 and Bob.

 

There was significant replication of XMRV

in CCR3 and Bonzo

although it is much lower

when compared to

DU145, A549 and LNCaP.

 

 

CONCLUSION:

 

XMRV replication

was observed in

GHOST cells that express CD4, and

each of the chemokine receptors

ranging from CCR1- CCR8 and Bob

suggesting that

infectivity in hematopoietic cells

could be mediated by use of these receptors.

 

 

PMID: 21896167

 

 

Samenvatting:

http://www.virologyj.com/content/8/1/423/abstract

 

Volledige tekst:

http://www.virologyj.com/content/pdf/1743-422X-8-423.pdf