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Lezing Mikovits:

Methoden voor

het opsporen van


in het bloed

(NYAS, 2011)








Judy Mikovits heeft onlangs een presentatie verzorgd over XMRV-opsporingsmethoden

in het kader van een lezingenserie over pathogenen (ziekteverwekkers) in de bloedvoorraad,

georganiseerd door The New York Academy of Sciences (29 maart 2011).


Relevant is vooral dat de variatie van "XMRV" groter blijkt te zijn dan gedacht.


Een samenvatting van de presentatie van Mikovits is hieronder weergegeven.

Onderaan deze pagina kunt U de video-opname van de presentatie (in drie delen) bekijken.






Disease Associations of XMRV and MLV-Related Viruses


Judy A. Mikovits, PhD,

Whittemore Peterson Institute for Neuro-Immune Disease



A new human retrovirus first associated with QQ variant RNaseL

a gene in the hereditary prostate cancer gene locus, in prostate cancer in 2006.


Detection of integration sites in unmanipulated prostate tissue;

demonstration of neutralizing antibody and

in situ hybridization for XMRV,

supports XMRV as a human retroviral infection associated with prostate cancer.


Although in the absence of direct sequencing

it cannot be rule out the reactivity can be to related MLV viruses.


In 2009 using

a classical virology approach of viral isolation and transmission,

electron micropscopy, serology and PCR,

Lombardi et al demonstrated

the first isolation of XMRV

from blood from patients with chronic fatigue syndrome (CFS)

predominately from the west coast of the United States.


In 2010,

Lo et al. extended these studies

by detecting nucleic acids of MLV-related variants

in the peripheral blood mononuclear cells of CFS

from the northeastern United States

suggesting additional strains capable of infecting humans exist.


In a study of 300 CFS patients, 13 developed lymphoproliferative disorders.


Of those tested,

11/11 were positive for XMRV and 9/9 positive for clonalTCR gamma rearrangements.


Spontaneous development of four immortalized B cells lines

occurred during culture of cells from CFS patients.


Three developed from B cells

isolated from the peripheral blood

(two of whom had B cell lymphoma) and

one from a bone marrow biopsy.


The B cell lines have a mature CD20+, CD23+ phenotype and produce infectious XMRV.


Virus production occurred

despite extensive hypermutation of the proviruses in these cells by APOBEC3G.



XMRV infection may accelerate the development of B cell malignancies

by either indirect chronic stimulation of the immune system and/or by direct infection

of the B-cell lineage.


Since viral load in peripheral blood is low,

these data suggest that B cells in tissues

such as spleen and lymph nodes

could be an in vivo reservoir for XMRV.


We have also identified

an inflammatory cytokine and chemokine signature

that distinguishes XMRV infected CFS patients from healthy controls

with 94% sensitivity and specificity.


Monitoring immune dysfunction

affords the opportunity to begin to understand

the pathogenesis of XMRVs.


In addition to these data,

recent advances in developing tests for detection and characterization of XMRV–variants

will be also be discussed




Strategies for Detection of XMRV in Blood.

Judy Mikovits


Pathogens in the blood supply.

Emerging Infectious Diseases & Microbiology Discussion Group.

New York Academy of Sciences.

March 29th, 2011.




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deel 3: vragen en antwoorden